In the era of high throughput genomics, investigators are frequently presented with lists of mutated or otherwise altered genes implicated in disease. Numerous resources exist to help form hypotheses about how such genomic events might be targeted therapeutically or prioritized for drug development. However, utilizing these resources typically requires tedious manual review of literature and knowledge bases. No informatics tools currently exist which mine these resources and provide a simple interface for searching lists of genes against the existing compendia of both known or predicted drug-gene interactions and potentially druggable genes. The Drug-Gene Interaction database (DGIdb) addresses this challenge. Drug-gene interactions have been mined from existing databases and literature to populate DGIdb. Similarly, genes have been categorized as potentially druggable according to membership in selected pathways, molecular functions and gene families. Currently, DGIdb contains 2,611 genes and 6,307 drugs involved in 14,144 drug-gene interactions, and 6,761 genes that belong to one or more of 39 potentially druggable gene categories. Users can enter a gene or list of genes to retrieve all known or potentially druggable genes in that list. Results can be filtered by source, interaction type, or treatment type and are sorted by source trust level. Our goal was to create a user-friendly search tool and comprehensive database of genes that have the potential to be druggable with a particular focus on cancer. DGIdb can be accessed programmatically or through a web-based interface at dgidb.org.